Building on post #6 rather than restating it.
Visible particulate is a strong signal in the other direction: it is a reason to stop and ask rather than to filter and proceed.
I have left out the parts I could not verify.
27 likes 7mo
Topic summary
Storing lyophilised material long term: what governs the shelf life
This is a generated summary. It shows the 9 most-liked posts from a topic of 91, in their original order, with the accepted answer included where one exists. It is a reading aid and it will miss nuance — the full topic is the record.
Picking up post #20: that is the part I would want checked first.
An observation about storing lyophilised material long term that I cannot explain and am posting anyway, on the principle that unexplained observations are more useful public than private.
31 likes 7mo
Adding the measurement that post #24 says would settle it.
Container choice matters: the material of the vial, the rubber closure, the fill volume all affect how quickly the contents degrade. Smaller fill volumes lose less to adsorption. Glass vials lose less to leaching than plastics.
I am reporting what happened, not recommending it.
23 likes 6mo
Lyophilised material is far more stable than the same material in solution. That is why suppliers ship it dry, and it is the reason to reconstitute only what will be used.
32 likes 6mo
On storing lyophilised material long term, the part that usually goes wrong is that the question is asked as though it has one answer. It has a range, and the width of the range is the interesting bit.
If you can post the two or three numbers you are working from, several people here will check the arithmetic rather than argue about the conclusion.
26 likes 6mo
i.guerrero, post #53: Reconstituted material carried in a bag for a day is a temperature excursion even in a cool climate. Where that matters is cumulative exposure over weeks rather than a single afternoon. Worth saying I have only my own numbers here, and n is small. Go to post
Posting my storing lyophilised material long term numbers with the method attached so they can be discounted properly. Uncontrolled, unblinded, and collected by someone who wanted a particular answer.
Post #69 and I disagree about the size of the effect, not about the direction.
Protecting from light: amber vials or opaque containers reduce light exposure. If you are storing for long periods and light matters, this is worth doing. For short-term prepared solutions, ordinary storage away from direct sunlight is usually adequate.
It is worth checking rather than assuming, which costs nothing.
32 likes 5mo
a.finnegan_rd, post #7: Building on post #6 rather than restating it. Visible particulate is a strong signal in the other direction: it is a reason to stop and ask rather than to filter and proceed. I have left out the parts I could not verify. Go to post
Visible particulate is a strong signal in the other direction: it is a reason to stop and ask rather than to filter and proceed.
Read the full topic (91 posts)
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