Quantitation at home is the part that does not survive scrutiny. Precision adequate to distinguish 96 from 98 per cent requires instrumentation and calibration that a kit does not have.
One case, stated as one case.
Quantitation at home is the part that does not survive scrutiny. Precision adequate to distinguish 96 from 98 per cent requires instrumentation and calibration that a kit does not have.
One case, stated as one case.
A test that gives a yes or no on the presence of peptide bonds is answering a genuinely useful question if that is the question you had. It is not a purity assay and cannot be read as one.
I read post #3 twice before replying, because I had assumed the opposite.
The honest cost comparison is a home kit against a single independent submission. For most people the second answers the question and the first answers a different, smaller one.
I have seen it go both ways, which is why I hedge.
Adding the measurement that post #12 says would settle it.
False positives and false negatives: no test has 100% sensitivity and 100% specificity. A negative result does not guarantee absence; a positive result does not guarantee presence. The predictive value depends on prevalence.
Adding the measurement that post #45 says would settle it.
Reconstitution behaviour is a legitimate home observation. A cake that will not dissolve, a solution that hazes, visible particulate — all are real information and all are free.
Second-hand, so weight it accordingly.
Read the full topic (51 posts)
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Reflectance and photometric devices: precision in practice — a second dataset
Reflectance and photometric devices: precision in practice — a second dataset — setting out what I have, and where I think it stops being reliable. Posting a small dataset on Reflectance and photometric…
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+75 | 82 | 17k | 12h |
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Coming back to: Why quantitation is the hard part, not detection
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+86 | 92 | 54k | 8mo |
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Coming back to: Lateral-flow and colorimetric approaches: the specificity problem
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Why quantitation is the hard part, not detection
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+11 | 15 | 27k | 12mo |
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Reading a home test result without over-claiming
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+124 | 137 | 19k | 7h |
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LOD and LOQ: definitions people get wrong
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2 | 43k | 8mo | |
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An inter-laboratory comparison on one lot, tabulated — what changed since
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+24 | 28 | 3.3k | 7mo |
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Follow-up: Why this community will not facilitate a group purchase
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2 | 19k | 18mo | |
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Second pass at: A payment that went through twice: how it was resolved
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2 | 1.1k | 14mo | |
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Revisiting: Spike-and-recover accuracy for a peptide assay
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